| 產品編號 | bs-48022P |
| 英文名稱 | Recombinant human TPO protein (Active, HEK293) |
| 中文名稱 | 重組人血小板生成素 (活性的, Tag free) |
| 別 名 | Thrombopoietin,C-mpl ligand,Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor,Myeloproliferative leukemia virus oncogene ligand,ML,MGDF,THPO, TPO-HUMAN. |
| 檢測分子量 | 80-85 |
| 性 狀 | Lyophilized |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | Human |
| 序 列 | 22-353/353 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | <0.5 EU/μg as determined by LAL. |
| 表達系統(tǒng) | HEK293 cell |
| 活性 | Yes |
| 標簽 | Tag free |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after r |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Recombinant human thrombopoietin expressed in engineered human cells. TPO belongs to the EPO/TPO protein family. TPO protein is an 80 to 85 kDa monomeric glycoprotein. TPO is primarily produced in the liver and regulates the formation of megakaryocytes and platelets. C terminal domain glycosylation is thought to be important for the secretion of TPO from cells and for survival of TPO in the circulation. This recombinant human TPO is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of human Mo7e cells. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. SWISS: P40225 Gene ID: 7066 |
| 產品圖片 |
The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
