| 產(chǎn)品編號(hào) | C05-03041 |
| 英文名稱 | Stripping buffer |
| 中文名稱 | 膜再生液 |
| 別 名 | Membrane Stripping; Western抗體洗脫液; Western一抗二抗去除液; Western Blot 膜再生液; WB膜再生液; Western膜再生液; WB 膜再生液; 剝離液; 剝脫液; WB一抗二抗去除液; 蛋白印跡膜再生液; |
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Specific References (1) | C05-03041 has been referenced in 1 publications.
[IF=4.638] Shang Kun. et al. SLC27A2 mediates FAO in colorectal cancer through nongenic crosstalk regulation of the PPARs pathway. BMC CANCER. 2023 Dec;23(1):1-11
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| 保存條件 | Store at RT. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Western Blot Stripping Buffer is a gentle method for breaking antibody-antigen interactions to allow nitrocellulose and PVDF membranes to be reprobed several times using different antibodies, saving time and conserving samples. Ideal for use with chemiluminescent substrates. 使用膜再生液可以對(duì)同一張膜進(jìn)行多次 Western Blot 檢測(cè)。在室溫條件下，將用過(guò)的膜浸泡在膜再生液 10-60 分鐘，可在不影響抗原蛋白的情況下清除膜上結(jié)合的抗體。隨后，可以使用不同的抗體進(jìn)行下一輪 Western Blot 實(shí)驗(yàn)。適宜從少量樣品多次檢測(cè)不同蛋白質(zhì)。即使樣品量并不匱乏，采用這一策略多次檢測(cè)同一 張膜上的蛋白，能省去給藥處理、蛋白電泳和膜轉(zhuǎn)移等耗費(fèi)性步驟。而且可以消除重新上樣而帶來(lái)的誤差，使可比性更強(qiáng)。 |
