產(chǎn)品編號 | bsm-33184M |
英文名稱 | Mouse Anti-AmCyan antibody |
中文名稱 | AmCyan藍色熒光蛋白單克隆抗體 |
別 名 | AmCyan Tag; AmCyan-Tag; 野生型天竺葵青色熒光蛋白; 藍色熒光蛋白AmCyan標簽; 藍色熒光蛋白標簽; |
產(chǎn)品類型 | 標簽抗體 |
研究領(lǐng)域 | 細胞生物 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 5F9 |
交叉反應(yīng) | Recombinant protein |
產(chǎn)品應(yīng)用 | WB=1:1000-5000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant AmCyan |
亞 型 | IgG |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation. SWISS: N/A Gene ID: N/A |
產(chǎn)品圖片 |
Sample:
Lane 1: AmCyan-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33184P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti- AmCyan-Tag (bsm-33184M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
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1、抗體溶解方法 | |
2、抗體修復(fù)方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |